Figure 3

Hyperglycemia reduced macropinocytosis while neither osmotic stress nor high insulin altered uptake. A, MΦs were cultured for 48 h at the glucose concentrations indicated and FITC-albumin uptake was measured by flow cytometry. B, MΦs were cultured for 48 h in low glucose with mannitol at the concentrations indicated. FITC-albumin uptake was measured by flow cytometry. C, MΦs were cultured for 48 h in low glucose with insulin at the concentrations indicated. FITC-albumin uptake was measured by flow cytometry. Results are representative of three independent experiments ± SEM. *: p < 0.05 from low glucose controls.