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Table 1 Primers used for cloning of CRS peptides and for qRT-PCR assays

From: Regional variations in Paneth cell antimicrobial peptide expression along the mouse intestinal tract

Primers used for cloning of CRS peptides

Product

Sense

Antisense

CRS

5'aaggctctg(c/g)tcttcaagat3'

5'tg(c/g)tcttcaagatg(t/c)agccc3'

5'ccatcctaatacgactcactatagggc3'

CRS4C-4

5'gtgagtgctggactcagcc3'

5'gattgcatttgcagctcggg3'

CRS4C-6

5'cctaggaaagcctccccagt3'

5'ttttgtcatgcaattgcacc3'

Primers used for qRT-PCR assays

CRS1C

5'caccacccaagctccaaatacacag3'

5'atcgtgaggaccaaaagcaaatgg3'

CRS4C

5'gcatggaatctgggtcaagataac3'

5'agaaggaagagcaatcaaggctaag3'

Crypt1

5'tcaagaggctgcaaaggaagagaac3'

5'tggtctccatgttcagcgacagc3'

Crypt4

5'gctgtgtctatctcctttggaggc3'

5'cgtattccacaagtcccacgaac3'

mLys

5'ggctggctactatggagtcagcctg3'

5'gcattcacagctcttggggttttg3'

pLys

5'gccaaggtctaacaatcgttgtgagttg3'

5'cagtcagccagcttgacaccacg3'

sPLA2

5'aggattcccccaagatgccac3'

5'cagccgtttctgacaggagttctgg3'

TCF-4

5'cggctcactccacagctcaaag3'

5'cgagaatctggttgatggctgc3'

NOD2

5'cgacatctcccacagagttgtaatcc3'

5'ggcacctgaagttgacattttgc3'

MMP7

5'ttcaagagggttagttgggggactg3'

5'ttgtcaaagtgagcatctccgcc3'

Reg3g

5'cctcaggacatcttgtgtctgtgctc3'

5'tccacctctgttgggttcatagcc3'

Crypt4 C57BL/6

5'ccaggggaagatgaccaggctg3'

5'tgcagcgacgatttctacaaaggc3'

  1. Note that two gene-specific sense primers were used to clone CRS cDNA.