Figure 6

Activation of peritoneal MC induces C5aR upregulation. Peritoneal MC from BALB/c mice were purified by a negative selection technique and cultured with ionomycin (A) or Ag (DNP-albumin, following a 24 h preincubation period with IgE anti-DNP) (B) for different periods as indicated. Subsequently, MC were stained by indirect immunofluorescence and analyzed by FACS. Gates were set on CD117⁺ MC (A, B). In (C), untreated peritoneal lavage cells were stained by indirect immunofluorescence and gates set on F4/80⁺ macrophages. Filled histograms indicate staining with rat IgG1 control mAb, open histograms (solid lines) with mAb 1240 against murine C5aR, and open histograms (dotted lines) with mAb 1G4 against murine C3aR. One representative experiment each of 3 is shown.