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Figure 2 | BMC Immunology

Figure 2

From: Coexpression of GM-CSF and antigen in DNA prime-adenoviral vector boost immunization enhances polyfunctional CD8+ T cell responses, whereas expression of GM-CSF antigen fusion protein induces autoimmunity

Figure 2

Influence of GM-OVA on the generation of DC (A) and stimulation of OT-I specific T-cells (B). A) BM-derived monocytes were cultured for 8 days in the presence of GM-OVA or recombinant GM-CSF (5 ng/ml each) and IL-4 (1 ng/ml). The CD11c+ cells were analysed for the expression of CD80, CD83, and CD86 surface markers before (left panel) and after addition of LPS (right panel). B) Splenocytes of TCR-transgenic OT-I mice were labelled with CFSE and incubated for 72 h in the presence of either ovalbumin, GM-OVA or OT-I peptide at the indicated final concentration. The addition of recombinant GM-CSF and ovalbumin as separate proteins did not induce proliferation (data not shown).

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BMC Immunology

ISSN: 1471-2172

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