Figure 2
From: CD43 signals induce Type One lineage commitment of human CD4+ T cells
CD43 and TcR signals induce the transcription of IFNγ in CD4+ and IL-4 in CD8+ T cells. T cells were treated for 6 h as indicated in Figure 1. Total RNA was extracted from CD4+ (panel A and C) or CD8+ T cells (panel B and D) and subjected to real time RT-PCR analysis for IFNγ (panels A and B) or IL 4 (panels C and D) mRNA contents. A representative of at least 3 independent experiments is shown. Each point is the mean of duplicates which differed by <2%.