Figure 1

CD43 and TcR signals induce the expression of IFNγ in CD4⁺ T cells. CD4⁺ or CD8⁺ T cells were used freshly isolated or after differentiation for one week under T1 or T2 polarizing conditions. Panel A shows the simultaneous analysis of IL-4 and IFNγ expression in freshly isolated cells. In Panel B we show the comparison of IFNγ expression in freshly isolated cells (left panel), and in T1 and T2 differentiated populations. Cells were left non-stimulated or were stimulated for 12 h by antibody ligation of CD3 (TcR), CD43 or the simultaneous ligation of both molecules. Cells were collected and stained with FITC-labelled anti-IFNγ antibody and analyzed by flow cytometry. Representative experiments, out of at least three are shown, using CD4⁺ or CD8⁺ T cells from the same donor.