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Figure 7 | BMC Immunology

Figure 7

From: Differential role of ICAM ligands in determination of human memory T cell differentiation

Figure 7

ICAM ligands induce distinct functional properties in ex vivo generated memory CD4+ T cells. (A) Analysis of transmigrated memory cell subsets after 21 days of stimulation with CD3, CD3/CD28, ICAM-1/CD3/CD28, ICAM-2/CD3/CD28, or ICAM-3/CD3/CD28. Frequency of subset specific transmigration is presented on the axis. Inset is display of the CD4+CD45RO+CD11aDim and CD4+CD45RO+CD11aBr populations on CD27 expression. (B) Intracellular activation of phospho-JNK, phospho-p38, and phospho-p44/42 upon IP-10, MIP1β, or MIP3α stimulation (100 ng, 15 min) CD4+ T cells stimulated for 21 days with CD3/CD28, ICAM-1/CD3/CD28, ICAM-2/CD3/CD28, or ICAM-3/CD3/CD28. Fluorescent intensities are shown relative to CD3/CD28 and normalized to a Log2 scale. (C) Phospho-subset frequency for phospho-p44/42 and phospho-JNK populations for the MIP3α stimulated cohort as described above. Experiments were reproduced with cells from two different donors. Transmigration experiments were repeated in duplicates with material from two donors. (D) Quantitation of percent phospho-p44/42/phospho-JNK cells of the 21 day differentiated CD4+CD45RO+CD11aBr subset as stimulated by MIP3α from two different donors.

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BMC Immunology

ISSN: 1471-2172

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