Figure 2

Granulysin and perforin can be sequentially added to induce enhanced intracellular listeriolysis. L. innocua-challenged DC were pulsed for 15 minutes with 2.5 μM granulysin and after medium replacement were then treated at indicated time points with perforin at a concentration of 2.5 kU/ml at 37°C for an overall experimental duration of 180 minutes. Alternatively, L. innocua-challenged DC were pretreated for 15 minutes with perforin and after medium replacement were then incubated with granulysin at a concentration of 2.5 μM at 37°C for 180 minutes. After the incubation, the cells were lysed in ice-cold water and Listeria viability was calculated from bacterial growth curves. Mean values and SD of four independent experiments are presented.