Your privacy, your choice

We use essential cookies to make sure the site can function. We also use optional cookies for advertising, personalisation of content, usage analysis, and social media.

By accepting optional cookies, you consent to the processing of your personal data - including transfers to third parties. Some third parties are outside of the European Economic Area, with varying standards of data protection.

See our privacy policy for more information on the use of your personal data.

for further information and to change your choices.
Skip to main content
Figure 3 | BMC Immunology

Figure 3

From: Structure function analysis of SH2D2A isoforms expressed in T cells reveals a crucial role for the proline rich region encoded by SH2D2A exon 7

Figure 3

Differential Lck phosphorylation of variant TSAd molecules. A. Tyrosine phosphorylation of TSAd variants in Jurkat T cells: Jurkat T cells transiently transfected with pEF-HA or pEF-HA-SH2D2A-1-5 cDNAs, were stimulated (+) with anti-CD3 (OKT3) mAbs for 2.5 min or left unstimulated (-), lysed and subjected to immunoprecipitation with anti-TSAd Abs (TSAd IP). The precipitates were separated by SDS-PAGE and immunoblotted with anti-phoshotyrosine (pY) mAbs (upper panel) and anti-HA mAbs (lower panel). Relative level of tyrosine phosphorylation of TSAd variants is shown in the chart, and the relationships between the pY signal versus the HA signal of bands is normalised to that observed for the full length TSAd IP (SH2D2A-1) expressed in resting Jurkat T cells. B. Lck phosphorylates the SH2D2A-1, -2 and -3 variants of TSAd in 293T cells: 293T cells were transiently transfected with pEF-HA or pEF-HA-SH2D2A-1-5 cDNAs alone (-) or together (+) with pEF-Lck. The cells were lysed and treated as in A. C. Tyrosine phosphorylation of TSAd mutated for the four C-terminal tyrosines is attenuated when co-expressed with Lck in 293T cells. 293T cells were transiently transfected with pEF-HA, the pEF-HA-TSAd-4YF or pEF-HA-TSAd-d239–256 cDNAs together with pEF-Lck. The cells were lysed and treated as in A.

Back to article page

BMC Immunology

ISSN: 1471-2172

Contact us