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Table 1 IL-1β-induced TNF secretion is decreased in lipid-loaded macrophages

From: Decreased inducibility of TNF expression in lipid-loaded macrophages

 

TNF concentration (pg/mL)

Treatment

Experiment 1

Experiment 2

Experiment 3

LPDS

5.9

5.4

6.4

LPDS + IL-1β

14.2

165.3

23.0

Ac-LDL

5.5

5.6

5.4

Ac-LDL + IL-1β

8.4

54.5

14.6

Inhibition

41%

67%

36%

  1. Macrophages were incubated for 48 h in the presence of 5% LPDS ± 50 μg/mL ac-LDL. They were then rinsed with serum-free medium, and incubated for 24 h in serum-free medium with or without IL-1β (10 ng/mL). Culture medium was collected, centrifuged at 4000 g for 10 min, and aliquots of the supernatant were frozen for analysis of secreted TNF by ELISA.
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BMC Immunology

ISSN: 1471-2172

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