Figure 4

Activation of SPM in 50 μm vibratome sections from the spinal cord of rats with severe EAE. All cells which have been labeled by icv application of FE (green in A,C,E) are ED2-positive (B), OX6-positive (D), SILK6-positive (F). G, H: Almost all FE-labeled SPM express the CD40 molecule (CY3). I, J, K, L: FE-labeled SPM (I, K) express also the B7-1 (J) and the B7-2 (L) molecule as detected by immunofluorescence with anti-CD80, anti-CD86, and CY3.