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Figure 1 | BMC Immunology

Figure 1

From: Phorbol esters and CAMP differentially regulate the expression of CD4 and CD8 in human thymocytes

Figure 1

Northern blot analysis of the effect of PMA and cAMP on CD4 and CD8 mRNA levels in human thymocytes. Cells were incubated for 4 h at 37°C in culture media as described in the methods, in the absence (lane 1) or in the presence of 10 nM PMA (lane 2) or 1 mM dibutyryl cAMP plus 1 mM IBMX (lane 3), and the combination of both (lane 4). RNA was subsequently extracted and analyzed. The expression of the proto-oncogene c-Ha-ras served as an internal control.

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