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Figure 3 | BMC Immunology

Figure 3

From: Macrophages exposed continuously to lipopolysaccharide and other agonists that act via toll-like receptors exhibit a sustained and additive activation state

Figure 3

Comparative dose response curves for activation of IL-12, IL-1 and ELAM promoters. Pooled stable transfectants of RAW264 cells, with either the ELAM or IL-12 promoter driving firefly luciferase, cotransfected with the IL-1β promoter driving renilla luciferase, were cultured overnight as described in Materials and Methods. E. coli genomic DNA (bDNA), LPS or PAM3-CSK lipopeptide (PAM) were added at the concentrations indicated (LPS and PAM are in ng/ml, bDNA in μg/ml). After 8 hrs, the cells were harvested for determination of luciferase activity. Datapoints are the average of duplicate wells. The experiment is representative of two, and reiterates the pattern observed in 2 others with differences in design.

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BMC Immunology

ISSN: 1471-2172

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