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Figure 2 | BMC Immunology

Figure 2

From: Macrophages exposed continuously to lipopolysaccharide and other agonists that act via toll-like receptors exhibit a sustained and additive activation state

Figure 2

Time course of activation ofIL-6 and IL-1 promoters in RAW264 cells and the effect of a range of microbial agonists. Pooled stable transfectants with the IL-6 promoter driving firefly luciferase and the IL-6 promoter driving renilla luciferase were incubated overnight then stimulated with the agents shown for the time indicated prior to assay of luciferase activity. PMA3-CSK was added at 100 ng/ml, peptidoglycan at 10 μg/ml and E-coli genomic DNA (bDNA) at 10 μg/ml. A representative experiment of two is shown. Datapoints are the average of duplicate wells.

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BMC Immunology

ISSN: 1471-2172

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