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Table 2 Jurkat T-cells were treated with 10 nM NAI, 10 μM JNK-I or10 nM PKC-I for 2 h followed by induction with 162 nM PMA for 24 h.

From: Differential cytokine regulation by NF-κB and AP-1 in Jurkat T-cells

 

C

PMA

NAI

JNK-I

PKC-I

IL-6

11.7 ± 2.4

63.6 ± 11.9

3.3 ± 4.0*

2.7 ± 2.2**

39.0 ± 20.8

CXCL8

450 ± 139

50294 ± 19161

7411 ± 545**

3197 ± 1079*

23238 ± 2547

  1. Gene expression was determined using RT-qPCR. The Ct values were normalized against 18S. Statistical significance from the positive control (PMA) was determined using Student's t-test. Concentrations are given as fg/ml, values are presented as mean ± SEM, n = 3.
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BMC Immunology

ISSN: 1471-2172

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